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Saturday, April 25, 2020 | History

2 edition of isolation and characterization of dex1, a pollen wall mutant of Arabidopsis thaliana found in the catalog.

isolation and characterization of dex1, a pollen wall mutant of Arabidopsis thaliana

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Published .
Written in English

    Subjects:
  • Arabidopsis thaliana -- Genetics,
  • Arabidopsis thaliana -- Pollen

  • Edition Notes

    Statementby Dawn Paxson Sowders
    The Physical Object
    Paginationv, 215 leaves :
    Number of Pages215
    ID Numbers
    Open LibraryOL15314761M


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isolation and characterization of dex1, a pollen wall mutant of Arabidopsis thaliana by Dawn Paxson Sowders Download PDF EPUB FB2

To identify factors that are required for proper pollen wall formation, we have characterized the T-DNA-tagged, dex1 mutation of Arabidopsis, which results in defective pollen wall pattern formation. This study reports the isolation and molecular characterization of DEX1 and morphological and ultrastructural analyses of dex1 plants.

DEX1 encodes a novel plant protein that is. Isolation and characterization of a male-sterile Arabidopsis thaliana mutant defective in pollen wall formation. 8TH INTERNATIONAL CONFERENCE ON ARABIDOPSIS RESEARCH AT3G   Aliphatic alcohols naturally exist in many organisms as important cellular components; however, their roles in extracellular polymer biosynthesis are poorly defined.

We report here the isolation and characterization of a rice (Oryza sativa) male-sterile mutant, defective pollen wall (dpw), which displays defective anther development and degenerated pollen grains with an irregular by: mutation of Arabidopsis, which results in defective pollen wall pattern formation.

This study reports the isolation and molecular characterization of DEX1 and morphological and ultrastructural. In angiosperms, pollen wall pattern formation is determined by primexine deposition on the microspores. Here, we show that AUXIN RESPONSE FACTOR17 (ARF17) is essential for a pollen wall mutant of Arabidopsis thaliana book formation and pollen development in Arabidopsis (Arabidopsis thaliana).

The arf17 mutant exhibited a male-sterile phenotype with normal vegetative growth. ARF17 was expressed in microsporocytes and Cited by: Dawn Paxson-Sowders, The Isolation and Characterization of dex1, a Pollen Wall Mutant of Arabidopsis thaliana (Ph.D., May ). Trinity Zang, Overexpression and Characterization of Glyoxalase II Isozymes from Arabidopsis thaliana (M.S., August ).

Glycolysis is a primary metabolic pathway whose main function is to oxidize hexoses to generate ATP, reducing power and pyruvate, and to produce precursors for anabolism (Plaxton, ).The major substrates fuelling glycolysis in plants are Suc and starch (Plaxton, ).Both metabolites are subjected to large diurnal changes in their concentrations that need to be coordinated with the rate of Cited by:   The precise structure of the sporopollenin polymer that is the major constituent of exine, the outer pollen wall, remains poorly understood.

Recently, characterization of Arabidopsis thaliana genes and corresponding enzymes involved in exine formation has demonstrated the role of fatty acid derivatives as precursors of sporopollenin building units.

Isolation and Genetic Analysis of the dpw Mutant. To identify rice genes that are important for normal anther and pollen development, we used 60 Co γ-ray radiation to generate a rice mutant library in the background, which is a cultivar of O.

sativa ssp japonica (Liu et al., ; Chen et al., ; Li et al., ; Wang et al., ).We isolated the dpw mutant by its complete male Cited by: Molecular Cell Biology of Pollen Walls. notype of the dex1 mutant suggests that DEX1 may be a component of the.

wall synthesis in Arabidopsis thaliana. Proc Natl Acad Sci USA –   After meiosis, the four microspores are enwrapped by callose to form a tetrad. The pollen-wall pattern is determined at tetrad stage. In contrast, the intine is synthesized by the microspore itself.

Many genes have been identified from male-sterile mutants in Arabidopsis thaliana and rice during recent years. The majority of these genes are Cited by: 9.

The tapetal cells provide precursors for sporopollenin formation. Pollen lacking the exine layer, such as that produced by the Arabidopsis male sterility 2 (Aarts et al., ; Chen et al., ) and no exine formation 1 mutants (Ariizumi et al., ) and the rice defective pollen wall mutant (Shi et.

Sporopollenin fortifies the outer wall (exine) of pollen grains, forming a durable casing around the male gametophyte. The surfaces of pollen grains often appear intricately decorated due to species-specific sculpturing of sporopollenin present in their outer walls, commonly assuming a regular hexagonal pattern, as in Arabidopsis thaliana (Arabidopsis) (Fig.

1A and B).Cited by:   The clustering of sporopollenin synthesis‐related genes and a sporopollenin transport‐related gene agrees with the observed regulation of exine formation; sporopollenin synthesis and transport have been previously shown to be spatially and temporally regulated and to work together in pollen wall development.

In Arabidopsis, lap3 mutant Cited by:   Pollen is produced within the anther, and covered by the specialized outer envelope, pollen wall. Although the morphology of pollen varies among different plant species, the pollen wall is mainly comprised of three layers: the pollen coat, the outer exine layer, and the inner intine by: In flowering plants, ideal male reproductive development requires the systematic coordination of various processes, in which timely differentiation and degradation of the anther wall, especially the tapetum, is essential for both pollen formation and anther dehiscence.

Here, we show that OsGPAT3, a conserved glycerolphosphate acyltransferase gene, plays a critical role in regulating anther Cited by: 2. Our work provides evidence for the involvement of a DOF family TF in phenylpropanoid metabolism.

In Arabidopsis thaliana, AtDOF4;2 negatively affects flavonoid accumulation under stress and its overexpression in the tapetum leads to the formation of flavonol‐free pollen by: For annuals, such as Arabidopsis thaliana that flower once, set seed and die, the timing is obviously extremely important.

It is now well known that flowering in Arabidopsis is induced by long-day (LD) 50 ßCAB International The Molecular Biology and Biotechnology of. Plant Developmental Biology - Biotechnological Perspectives Volume 1 Editors Prof. Eng-Chong Pua New Era College Jalan Bukit Kajang Selangor Malaysia [email protected] Dr.

Michael R. Davey Plant and Crop Sciences Division School of Biosciences University of Nottingham Sutton Bonington Campus Loughborough LE12 5RD UK [email protected]. are: total DNA isolation and characterization, G+C composition, PCR amplification.

of rDNA linked with denaturing gradient gel electrophoresis (DGGE), PCR amplification of functional genes, rRNA sequences and in situ hybridization. of rRNA oligonucleotide probes [].

Composition and Technological Properties of Prebiotic Oligosaccharides.